In Vitro Antimicrobial and Haemolytic Studies of Bambusa arundinaceae leaves

Article history: Received on: 16/01/2013 Revised on: 06/03/2013 Accepted on: 04/04/2013 Available online: 27/04/2013 Bambusa arundinacea locally known as Bans or bamboo, a perennial fastest growing plant. This plant is medicinally much famous. The biological activities of this plant were carried out by using standard procedures. The aim of this work was to investigate the in vitro antimicrobial and haemolytic activities of Bambusa arundinaceae leaves. The GC-MS analysis revealed that the major components determined in n-hexane extract were as n-Nonane (17.14%), 2,2,3-Trimethylbutane (5.85%), Tridecane (5.42%), and 3,4,5,6-Tetramethyloctane (5.32%). The disc diffusion and modified resazurin microtitre-plate assays were used to evaluate the inhibition zones (IZ) and minimum inhibitory concentration (MIC) of B. arundinaceae leaves extracts. The plant extracts showed considerable antimicrobial activity. The heamolytic activity against human blood erythrocytes (RBCs) was studied and the % lysis of RBCs was found to be in the range of 1.03 to 4.81. The results of the present study confirmed the considerable antimicrobial and haemolytic activities of the plant Bambusa arundinaceae.